Alternative methods to animal studies, often referred to as alternative methods, non-animal methods, or animal-free methods, are approaches used to replace, reduce, or refine the use of animals in scientific research and testing. These methods aim to achieve scientific goals while minimizing harm to animals and providing more ethical, humane, and sometimes more accurate models for studying human biology and disease. Here are some key alternative methods:
- Invitro ocular irritation test
- Skin irritation test
- CAM test (Angiogenesis)
- C. elegans (aging, age-related diseases, mechanisms of longevity, and drug screening)
- Zebrafish for cancer study
- Mosquito larvicidal activity
Future Directions
Advancements in technologies such as organ-on-a-chip, computational modelling, and stem cell research continue to drive innovation in alternative methods. These approaches hold promise for reducing reliance on animal studies while advancing biomedical research and ensuring human and environmental safety.